Anti-Nogo A 抗体 (ab62024)
Key features and details
- Rabbit polyclonal to Nogo A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nogo A antibody -
製品の詳細
Rabbit polyclonal to Nogo A -
由来種
Rabbit -
特異性
ab62024 is specific for Nogo A and Nogo E. -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).
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ポジティブ・コントロール
- WB: Human brain tissue lysate. IHC-P: Mouse brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).Can be blocked with Human Nogo A peptide (ab234893).
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).Can be blocked with Human Nogo A peptide (ab234893). |
ターゲット情報
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機能
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. -
組織特異性
Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific. -
配列類似性
Contains 1 reticulon domain. -
ドメイン
Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor. -
細胞内局在
Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. - Information by UniProt
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参照データベース
- Entrez Gene: 57142 Human
- Entrez Gene: 68585 Mouse
- Entrez Gene: 83765 Rat
- Omim: 604475 Human
- SwissProt: Q9NQC3 Human
- SwissProt: Q99P72 Mouse
- SwissProt: Q9JK11 Rat
- Unigene: 727710 Human
see all -
別名
- ASY antibody
- Foocen antibody
- Human NogoA antibody
see all
画像
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Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG
Predicted band size: 130 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml.
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Immunohistochemical (Formalin-fixed paraffin embedded sections) analysis of mouse Nogo A in brain tissue using ab62024 at 2.5µg/ml.
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Immunohistochemical analysis of 4% paraformaldehydefixed Nogo A in mouse brain tissue using ab8416 at 20µg/ml.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed rat brain tissue labelled Nogo A with ab62024 at 20µg/ml (green), followed by DAPI (blue) and phylloidin (red).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab62024 は 20 報の論文で使用されています。
- Peng K et al. A timeline of oligodendrocyte death and proliferation following experimental subarachnoid hemorrhage. CNS Neurosci Ther 28:842-850 (2022). PubMed: 35150055
- Feng XF et al. Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats. Front Pharmacol 13:851746 (2022). PubMed: 35559236
- Ullah HMA et al. Nogo-A Is Critical for Pro-Inflammatory Gene Regulation in Myocytes and Macrophages. Cells 10:N/A (2021). PubMed: 33572505
- Wang YY et al. Nogo-A aggravates oxidative damage in oligodendrocytes. Neural Regen Res 16:179-185 (2021). PubMed: 32788474
- Wang D et al. Combination of Electroacupuncture and Constraint-Induced Movement Therapy Enhances Functional Recovery After Ischemic Stroke in Rats. J Mol Neurosci N/A:N/A (2021). PubMed: 34101150